Journal: eBioMedicine

Article Title: Preclinical characterisation of the protective capacity of an anti-nucleoprotein hRSV monoclonal antibody

doi: 10.1016/j.ebiom.2025.106104

Figure Lengend Snippet: The humanised anti-N-hRSV mAbs demonstrate antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity activity in vitro . A. The percentage of antibody-dependent cellular cytotoxicity (ADCC) activity for each clone of the humanised anti-N-hRSV mAbs (clones P1-04H, P1-05D, P2-01A, and P2-01D) was measured using luciferase expression levels in Jurkat NFAT-luc FcγRIII cells incubated with the humanised anti-N-hRSV mAbs against N-hRSV. B. The percentage of complement-dependent cytotoxicity (CDC) activity for each clone was assessed by viability assay of HEp-2 cells infected with hRSV-GFP, treated with the four clones of the humanised anti-N-hRSV mAbs, followed by the addition of mouse purified complement. Three independent experiments were conducted for each ADCC and CDC assay (N = 3).

Article Snippet: After 30 min of antibody incubation, 100 μl of a 1:25 solution of mouse-purified complement (Rockland Immunochemical; cat #R.C201-0005) was added, bringing the final concentration in the well to 1:50, and the plate was incubated for 3 h at 37 °C with 5% CO 2 .

Techniques: Activity Assay, In Vitro, Clone Assay, Luciferase, Expressing, Incubation, Viability Assay, Infection, Purification, CDC Assay

Journal: Antioxidants

Article Title: Effects of Particle Size of Curcumin Solid Dispersions on Bioavailability and Anti-Inflammatory Activities

doi: 10.3390/antiox12030724

Figure Lengend Snippet: Uptake of C-SDs I–IV into RAW264 cells. Excitation wavelength: 488 nm, emission wavelength: 519 nm. C-SDs, curcumin solid dispersions; I, C-SDs I; II, C-SDs II; III, C-SDs III; IV, C-SDs IV. Scale bar in each image is 10 µm.

Article Snippet: Mouse leukemic monocyte macrophage (RAW264) cells were obtained from Japan Food Research Laboratories (Tokyo, Japan) and maintained in Dulbecco’s modified Eagle medium (DMEM D-6429; 4500 mg/L glucose, high pyruvate; Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin) in 100 mm plastic Petri dishes at 37 °C and 5% CO 2 .

Techniques:

Journal: Antioxidants

Article Title: Effects of Particle Size of Curcumin Solid Dispersions on Bioavailability and Anti-Inflammatory Activities

doi: 10.3390/antiox12030724

Figure Lengend Snippet: NO production in RAW264 cells. NO production and cell viability in LPS-stimulated RAW264 cells with C-SDs I-IV ( a,b,c p < 0.05 (Tukey’s posthoc test)). There is no statistically significant difference between groups in 0–1.0 µM. NO production in LPS-stimulated RAW264 cells (positive control, 0 µM) is 100%. C-SDs, curcumin solid dispersions; I, C-SDs I; II, C-SDs II; III, C-SDs III; IV, C-SDs IV.

Article Snippet: Mouse leukemic monocyte macrophage (RAW264) cells were obtained from Japan Food Research Laboratories (Tokyo, Japan) and maintained in Dulbecco’s modified Eagle medium (DMEM D-6429; 4500 mg/L glucose, high pyruvate; Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin) in 100 mm plastic Petri dishes at 37 °C and 5% CO 2 .

Techniques: Positive Control

Journal: Antioxidants

Article Title: Effects of Particle Size of Curcumin Solid Dispersions on Bioavailability and Anti-Inflammatory Activities

doi: 10.3390/antiox12030724

Figure Lengend Snippet: Gene expression levels in RAW264 cells. Gene expression levels (( A ) Il-1β , ( B ) Cox-2 , ( C ) Il-6 , ( D ) Mcp-1 , ( E ) Inos , ( F ) Tnf-α , ( G ) Nf-κb , ( H ) Tgf-β , ( I ) Ho-1 , ( J ) Cat , ( K ) Nrf2 ) in LPS-stimulated RAW264 cells with C-SDs I-IV (mean ± SE, n = 3. a,b p < 0.05 (Tukey’s posthoc test)). There is no statistically significant difference between groups in D–G,I,J. Cat , catalase; Cox-2 , cyclooxygenase-2; C-SDs, curcumin solid dispersions; Gapdh , Glyceraldehyde 3-phosphate dehydrogenase; Ho-1 , heme oxygenease 1; Il-1β , interleukin 1β; Il-6 , interleukin 6; Inos , inducible nitric oxide synthase; Mcp-1 , monocyte chemotactic protein 1; ND, not detected; Nf-κb , nuclear factor kappa b cells; Nrf2 , nuclear factor erythroid 2 related factor; Tgf-β , transforming growth factor β; Tnf-α , tumor necrosis factor α; I, C-SDs I; II, C-SDs II; III, C-SDs III; IV, C-SDs IV.

Article Snippet: Mouse leukemic monocyte macrophage (RAW264) cells were obtained from Japan Food Research Laboratories (Tokyo, Japan) and maintained in Dulbecco’s modified Eagle medium (DMEM D-6429; 4500 mg/L glucose, high pyruvate; Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin) in 100 mm plastic Petri dishes at 37 °C and 5% CO 2 .

Techniques: Gene Expression

Journal: Antioxidants

Article Title: Effects of Particle Size of Curcumin Solid Dispersions on Bioavailability and Anti-Inflammatory Activities

doi: 10.3390/antiox12030724

Figure Lengend Snippet: Secretion of inflammatory cytokines in RAW264 cells. Secretion of inflammatory cytokines (( A ) IL-1β, ( B ) IL-6, ( C ) MCP-1) in LPS-stimulated RAW264 cells with C-SDs I-IV (mean ± SE, n = 3. a,b,c,d p < 0.05 (Tukey’s posthoc test)). C-SDs, curcumin solid dispersions; Il-1β, interleukin 1β; IL-6, interleukin 6; MCP-1, monocyte chemotactic protein 1; ND, not detected; I, C-SDs I; II, C-SDs II; III, C-SDs III; IV, C-SDs IV.

Article Snippet: Mouse leukemic monocyte macrophage (RAW264) cells were obtained from Japan Food Research Laboratories (Tokyo, Japan) and maintained in Dulbecco’s modified Eagle medium (DMEM D-6429; 4500 mg/L glucose, high pyruvate; Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 U/mL penicillin and 100 μg/mL streptomycin) in 100 mm plastic Petri dishes at 37 °C and 5% CO 2 .

Techniques: